ssr_primer
Primer Details for TRINITY_DN69546_c2_g1_i1 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGGAAAAACGCCGCAGAA | GGACAGCGCCCTATTCTAGC | Start: 105 |
| Tm | 59.97 | 60.32 | End: 362 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | AAAAACGCCGCAGAAGCATT | GGACAGCGCCCTATTCTAGC | Start: 110 |
| Tm | 59.968 | 60.32 | End: 362 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | GGAGAGGACAGCACCAACAA | TGTGTTTGGGTAAACCGGGT | Start: 1729 |
| Tm | 59.891 | 59.742 | End: 1839 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | GAAGATTCGCAGCGCTTGAA | TTGTTGGTGCTGTCCTCTCC | Start: 1605 |
| Tm | 59.556 | 59.891 | End: 1748 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | GGAGAGGACAGCACCAACAA | TGTTTGGGTAAACCGGGTGG | Start: 1729 |
| Tm | 59.891 | 60.468 | End: 1837 |
| Size | 20 | 20 | 109 |
(C)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGGAAAAACGCCGCAGAA | GGACAGCGCCCTATTCTAGC | Start: 105 |
| Tm | 59.97 | 60.32 | End: 362 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | AAAAACGCCGCAGAAGCATT | GGACAGCGCCCTATTCTAGC | Start: 110 |
| Tm | 59.968 | 60.32 | End: 362 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | GGAGAGGACAGCACCAACAA | TGTGTTTGGGTAAACCGGGT | Start: 1729 |
| Tm | 59.891 | 59.742 | End: 1839 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | GAAGATTCGCAGCGCTTGAA | TTGTTGGTGCTGTCCTCTCC | Start: 1605 |
| Tm | 59.556 | 59.891 | End: 1748 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | GGAGAGGACAGCACCAACAA | TGTTTGGGTAAACCGGGTGG | Start: 1729 |
| Tm | 59.891 | 60.468 | End: 1837 |
| Size | 20 | 20 | 109 |
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