ssr_primer
Primer Details for TRINITY_DN69542_c4_g1_i3 |
|---|
| (G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGTCTCAAATCGAGCA | TTGCCACTCGGAAATCCCTC | Start: 76 |
| Tm | 60.036 | 60.035 | End: 266 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | TGGCGTGAAGATGTTCCTCC | GCTGCGCGATCTGGAGATAT | Start: 794 |
| Tm | 60.036 | 60.109 | End: 975 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | ATATCTCCAGATCGCGCAGC | CCCGACCAAATGGAACTGGA | Start: 956 |
| Tm | 60.109 | 59.962 | End: 1226 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | ATATCTCCAGATCGCGCAGC | ATGCCCGACCAAATGGAACT | Start: 956 |
| Tm | 60.109 | 59.96 | End: 1229 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
|---|
| Sequence | ACGACAGGACAGGTAAGGGA | GGAGGAACATCTTCACGCCA | Start: 702 |
| Tm | 59.886 | 60.036 | End: 813 |
| Size | 20 | 20 | 112 |
(A)10(ATAAATAAATAAATAAATAAATAAATAAAAAAATCTTTCTTGAAACTTGATGCGATATTGAGATAAGCTCCAAGTTTCTAGTGCCTTATCATTTTGGCAAACTTAAAAAATAAGAAAAGGCTCAACATTTTCGGAGACGCCAAAATATTTGACATTTTTTCTCTTTCTCCCAACTTATTCAGATTCTTTTTCCAATAAATGACGTTTCTGAGAATAGTGCTCCTAAATTTCAAGCATCTTTTAACACGACAGGACAGGTAAGGGAGATAAAGGTGTAAATGCGTGCATTAAACACTAGTGGTTTCTAAACTGATGAGTTTACAATTACCGTTTGCGGTGGCGTGAAGATGTTCCTCCGAGTTATTCCTAGAATCCGTTAACTTCTCTTCGCGTTGCATCATAACTAACCTAAAGCTTAAATAGTCGATCAAAGATTCCAATCCGTGGTTATCGTCGTTCAAAAATTCTCGCACCCACTCGATATGGTTCGTACGGAGGGATATCTCCAGATCGCGCAGCACTTGGGTCGAGGTGGCGTCGCTCACCATTTTCCGCTTCCTTGAACTGCGAGACGCCTTTGGATCTAAATATGTTCGTAGTTTGCTTAAATAATGTGCTGGCGTATCTTTCGCTTGAACTCTCTCCTGATCACAGATGATGTCCCATTTTTTTTCATTGTCGTACTGTTTCAAAAGTTTGGCCTTGTCCGGAGGTAAATCCATGGAGGCCAAAACTTTAGTAAACCGCCTTTCCAGTTCCATTTGGTCGGGCATCGGGAGCTTGGTCCGAATGCTAGCTCGAATGTTGGTCGAACGAGGGAATTCCACAGGGGGTGGAGATTCCATTTTTGAGGTTGAAGTATTCCCCATCTTATCAAGCACATGAAATTATCGAGATAAACAAAATTATCACTTCACAATCAGAGGAAGAACAAGAACACGAT)(AAAT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGTCTCAAATCGAGCA | TTGCCACTCGGAAATCCCTC | Start: 76 |
| Tm | 60.036 | 60.035 | End: 266 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | TGGCGTGAAGATGTTCCTCC | GCTGCGCGATCTGGAGATAT | Start: 794 |
| Tm | 60.036 | 60.109 | End: 975 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | ATATCTCCAGATCGCGCAGC | CCCGACCAAATGGAACTGGA | Start: 956 |
| Tm | 60.109 | 59.962 | End: 1226 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | ATATCTCCAGATCGCGCAGC | ATGCCCGACCAAATGGAACT | Start: 956 |
| Tm | 60.109 | 59.96 | End: 1229 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
|---|
| Sequence | ACGACAGGACAGGTAAGGGA | GGAGGAACATCTTCACGCCA | Start: 702 |
| Tm | 59.886 | 60.036 | End: 813 |
| Size | 20 | 20 | 112 |
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