ssr_primer
Primer Details for TRINITY_DN69525_c0_g1_i3 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTAAAGAGGCAACCCCGA | GCCGCGGCTCAATCTAAAAG | Start: 139 |
| Tm | 59.963 | 59.972 | End: 383 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CTTTTAGATTGAGCCGCGGC | CGTTGTCGTTGTCGTTGTCC | Start: 364 |
| Tm | 59.972 | 60.044 | End: 578 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | CTTTTAGATTGAGCCGCGGC | ATCAGTTCCATCTCGACGCC | Start: 364 |
| Tm | 59.972 | 59.897 | End: 511 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | CCGAATAGACCCGGGAAAGG | GCCGCGGCTCAATCTAAAAG | Start: 236 |
| Tm | 59.894 | 59.972 | End: 383 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | GGTTAAAGAGGCAACCCCGA | CCTTTCCCGGGTCTATTCGG | Start: 139 |
| Tm | 59.963 | 59.894 | End: 255 |
| Size | 20 | 20 | 117 |
(A)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTAAAGAGGCAACCCCGA | GCCGCGGCTCAATCTAAAAG | Start: 139 |
| Tm | 59.963 | 59.972 | End: 383 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CTTTTAGATTGAGCCGCGGC | CGTTGTCGTTGTCGTTGTCC | Start: 364 |
| Tm | 59.972 | 60.044 | End: 578 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | CTTTTAGATTGAGCCGCGGC | ATCAGTTCCATCTCGACGCC | Start: 364 |
| Tm | 59.972 | 59.897 | End: 511 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | CCGAATAGACCCGGGAAAGG | GCCGCGGCTCAATCTAAAAG | Start: 236 |
| Tm | 59.894 | 59.972 | End: 383 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | GGTTAAAGAGGCAACCCCGA | CCTTTCCCGGGTCTATTCGG | Start: 139 |
| Tm | 59.963 | 59.894 | End: 255 |
| Size | 20 | 20 | 117 |
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