ssr_primer
Primer Details for TRINITY_DN69521_c7_g1_i2 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGTGACTGAGATGGATCG | TGTCCTGGAACGAGTTGCTC | Start: 226 |
| Tm | 59.969 | 59.966 | End: 472 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
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| Sequence | CGGGTGACTGAGATGGATCG | GCTGGCTGGCTAAAGGGTAA | Start: 226 |
| Tm | 59.969 | 60.034 | End: 438 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
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| Sequence | CTGGGCCAGAATTCGGTCTT | TGTCCTGGAACGAGTTGCTC | Start: 195 |
| Tm | 60.035 | 59.966 | End: 472 |
| Size | 20 | 20 | 278 |
| Primer 4 details |
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| Sequence | TTCTGGGCCAGAATTCGGTC | TGTCCTGGAACGAGTTGCTC | Start: 193 |
| Tm | 60.035 | 59.966 | End: 472 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | CTGGGCCAGAATTCGGTCTT | GCTGGCTGGCTAAAGGGTAA | Start: 195 |
| Tm | 60.035 | 60.034 | End: 438 |
| Size | 20 | 20 | 244 |
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