ssr_primer
Primer Details for TRINITY_DN69474_c1_g1_i2 |
|---|
| (AG)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTGAGACCTACAAGTGC | TTTTAGGGAGGGAGAGGGGG | Start: 810 |
| Tm | 59.678 | 59.954 | End: 935 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | TCAGAACATTGGAGCCGTCG | ACAGTATGCCTCCCCTGGAT | Start: 1159 |
| Tm | 60.39 | 60.03 | End: 1344 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | CCCCCTCTCCCTCCCTAAAA | CGACGGCTCCAATGTTCTGA | Start: 916 |
| Tm | 59.954 | 60.39 | End: 1178 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TGGTTTCTCTGCAGGTGCTT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 124 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TTGGTTTCTCTGCAGGTGCT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 125 |
| Size | 20 | 20 | 116 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTGAGACCTACAAGTGC | TTTTAGGGAGGGAGAGGGGG | Start: 810 |
| Tm | 59.678 | 59.954 | End: 935 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | TCAGAACATTGGAGCCGTCG | ACAGTATGCCTCCCCTGGAT | Start: 1159 |
| Tm | 60.39 | 60.03 | End: 1344 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | CCCCCTCTCCCTCCCTAAAA | CGACGGCTCCAATGTTCTGA | Start: 916 |
| Tm | 59.954 | 60.39 | End: 1178 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TGGTTTCTCTGCAGGTGCTT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 124 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TTGGTTTCTCTGCAGGTGCT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 125 |
| Size | 20 | 20 | 116 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTGAGACCTACAAGTGC | TTTTAGGGAGGGAGAGGGGG | Start: 810 |
| Tm | 59.678 | 59.954 | End: 935 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | TCAGAACATTGGAGCCGTCG | ACAGTATGCCTCCCCTGGAT | Start: 1159 |
| Tm | 60.39 | 60.03 | End: 1344 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | CCCCCTCTCCCTCCCTAAAA | CGACGGCTCCAATGTTCTGA | Start: 916 |
| Tm | 59.954 | 60.39 | End: 1178 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TGGTTTCTCTGCAGGTGCTT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 124 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TTGGTTTCTCTGCAGGTGCT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 125 |
| Size | 20 | 20 | 116 |
(A)10taaaaacagaacataattaac(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTGAGACCTACAAGTGC | TTTTAGGGAGGGAGAGGGGG | Start: 810 |
| Tm | 59.678 | 59.954 | End: 935 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
|---|
| Sequence | TCAGAACATTGGAGCCGTCG | ACAGTATGCCTCCCCTGGAT | Start: 1159 |
| Tm | 60.39 | 60.03 | End: 1344 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
|---|
| Sequence | CCCCCTCTCCCTCCCTAAAA | CGACGGCTCCAATGTTCTGA | Start: 916 |
| Tm | 59.954 | 60.39 | End: 1178 |
| Size | 20 | 20 | 263 |
| Primer 4 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TGGTTTCTCTGCAGGTGCTT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 124 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | ACGTGAGGCCTGTTATGCAT | TTGGTTTCTCTGCAGGTGCT | Start: 10 |
| Tm | 59.747 | 59.817 | End: 125 |
| Size | 20 | 20 | 116 |
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