Primer Details for TRINITY_DN69445_c0_g1_i1 | |||
|---|---|---|---|
| (A)24: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTCAGCAAAGCCTCCAAA | TCAAGGGTCTAGACTTTAGATCCA | Start: 28 |
| Tm | 60.179 | 58.161 | End: 176 |
| Size | 20 | 24 | 149 |
| Primer 2 details | |||
| Sequence | TTCGTCAGCAAAGCCTCCAA | TCAAGGGTCTAGACTTTAGATCCA | Start: 27 |
| Tm | 60.179 | 58.161 | End: 176 |
| Size | 20 | 24 | 150 |
| Primer 3 details | |||
| Sequence | TTTCGTCAGCAAAGCCTCCA | TCAAGGGTCTAGACTTTAGATCCA | Start: 26 |
| Tm | 60.179 | 58.161 | End: 176 |
| Size | 20 | 24 | 151 |
| Primer 4 details | |||
| Sequence | GCAAAGCCTCCAAATGCGTA | TCAAGGGTCTAGACTTTAGATCCA | Start: 34 |
| Tm | 59.474 | 58.161 | End: 176 |
| Size | 20 | 24 | 143 |
| Primer 5 details | |||
| Sequence | TCGTCAGCAAAGCCTCCAAA | TCAAGGGTCTAGACTTTAGATCCAA | Start: 28 |
| Tm | 60.179 | 58.739 | End: 176 |
| Size | 20 | 25 | 149 |
