ssr_primer
Primer Details for TRINITY_DN69409_c3_g1_i4 |
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| (A)13tttaatgaagcattttttcctcttccttttttaaatt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATCGAGCATTTGGGCACT | ATGGAAGTCCTTGCTGCCTC | Start: 1283 |
| Tm | 60.035 | 60.035 | End: 1479 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
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| Sequence | TACGCGCAGTGCTCTTGTTA | TGTCCGTCAAGTCCAGCTTC | Start: 1970 |
| Tm | 60.039 | 59.966 | End: 2081 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
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| Sequence | TACGCGCAGTGCTCTTGTTA | CTGTCCGTCAAGTCCAGCTT | Start: 1970 |
| Tm | 60.039 | 59.966 | End: 2082 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
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| Sequence | AGACGATACTCCCCACCACA | ATGGAAGTCCTTGCTGCCTC | Start: 1358 |
| Tm | 59.959 | 60.035 | End: 1479 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
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| Sequence | CAGCTACAAAAACGCGCAGT | AGTGCCCAAATGCTCGATGA | Start: 1071 |
| Tm | 60.041 | 60.035 | End: 1302 |
| Size | 20 | 20 | 232 |
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