ssr_primer
Primer Details for TRINITY_DN69391_c2_g1_i3 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCAACCTGCCACTCATCA | ACGCTGGAGATGAGTTGCAA | Start: 1130 |
| Tm | 59.961 | 59.965 | End: 1304 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | GCACTTAAAGCCCTGGCCTA | ACAGGGGCCCTTGAGATACT | Start: 720 |
| Tm | 60.034 | 59.955 | End: 965 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | ATGAGCGCATCGGCAAATTC | GTCACACGTGTTTTGGCGAA | Start: 3855 |
| Tm | 59.97 | 59.903 | End: 4050 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | GATGAGCGCATCGGCAAATT | GTCACACGTGTTTTGGCGAA | Start: 3854 |
| Tm | 59.97 | 59.903 | End: 4050 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | GCCCAGCGAAAATCCAATCC | TAGGCCAGGGCTTTAAGTGC | Start: 603 |
| Tm | 59.897 | 60.034 | End: 739 |
| Size | 20 | 20 | 137 |
(TAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCAACCTGCCACTCATCA | ACGCTGGAGATGAGTTGCAA | Start: 1130 |
| Tm | 59.961 | 59.965 | End: 1304 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | GCACTTAAAGCCCTGGCCTA | ACAGGGGCCCTTGAGATACT | Start: 720 |
| Tm | 60.034 | 59.955 | End: 965 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | ATGAGCGCATCGGCAAATTC | GTCACACGTGTTTTGGCGAA | Start: 3855 |
| Tm | 59.97 | 59.903 | End: 4050 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | GATGAGCGCATCGGCAAATT | GTCACACGTGTTTTGGCGAA | Start: 3854 |
| Tm | 59.97 | 59.903 | End: 4050 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | GCCCAGCGAAAATCCAATCC | TAGGCCAGGGCTTTAAGTGC | Start: 603 |
| Tm | 59.897 | 60.034 | End: 739 |
| Size | 20 | 20 | 137 |
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