ssr_primer
Primer Details for TRINITY_DN69382_c3_g1_i3 |
|---|
| (T)27: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTGGGGGTCAGTCTACC | CTGGAGCACCCTAGCATGAC | Start: 157 |
| Tm | 60.035 | 60.179 | End: 285 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | GCTTTGGGGGTCAGTCTACC | TCTGGAGCACCCTAGCATGA | Start: 157 |
| Tm | 60.035 | 60.326 | End: 286 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | TTGGGGGTCAGTCTACCACT | CTGGAGCACCCTAGCATGAC | Start: 160 |
| Tm | 59.807 | 60.179 | End: 285 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | CGGTAATGCTTTGGGGGTCA | CTGGAGCACCCTAGCATGAC | Start: 150 |
| Tm | 60.323 | 60.179 | End: 285 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TTCCGGTAATGCTTTGGGGG | CTGGAGCACCCTAGCATGAC | Start: 147 |
| Tm | 60.324 | 60.179 | End: 285 |
| Size | 20 | 20 | 139 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTGGGGGTCAGTCTACC | CTGGAGCACCCTAGCATGAC | Start: 157 |
| Tm | 60.035 | 60.179 | End: 285 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | GCTTTGGGGGTCAGTCTACC | TCTGGAGCACCCTAGCATGA | Start: 157 |
| Tm | 60.035 | 60.326 | End: 286 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | TTGGGGGTCAGTCTACCACT | CTGGAGCACCCTAGCATGAC | Start: 160 |
| Tm | 59.807 | 60.179 | End: 285 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | CGGTAATGCTTTGGGGGTCA | CTGGAGCACCCTAGCATGAC | Start: 150 |
| Tm | 60.323 | 60.179 | End: 285 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TTCCGGTAATGCTTTGGGGG | CTGGAGCACCCTAGCATGAC | Start: 147 |
| Tm | 60.324 | 60.179 | End: 285 |
| Size | 20 | 20 | 139 |
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