ssr_primer
Primer Details for TRINITY_DN69317_c9_g2_i7 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGACTGGTTGCGAGAAGTT | ACGATGCGATGCGGATACTT | Start: 1968 |
| Tm | 59.896 | 59.968 | End: 2115 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | AACGACTGGTTGCGAGAAGT | ACGATGCGATGCGGATACTT | Start: 1967 |
| Tm | 59.896 | 59.968 | End: 2115 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | AGTCATTCCACTTGCAGCGA | TAATCGTGCCCGGAGTCATG | Start: 1665 |
| Tm | 59.965 | 59.896 | End: 1785 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | AGTCATTCCACTTGCAGCGA | GTAATCGTGCCCGGAGTCAT | Start: 1665 |
| Tm | 59.965 | 59.896 | End: 1786 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | CGGATGAGAACGGTGGAACA | TAATCGTGCCCGGAGTCATG | Start: 1638 |
| Tm | 60.038 | 59.896 | End: 1785 |
| Size | 20 | 20 | 148 |
(C)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGACTGGTTGCGAGAAGTT | ACGATGCGATGCGGATACTT | Start: 1968 |
| Tm | 59.896 | 59.968 | End: 2115 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | AACGACTGGTTGCGAGAAGT | ACGATGCGATGCGGATACTT | Start: 1967 |
| Tm | 59.896 | 59.968 | End: 2115 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | AGTCATTCCACTTGCAGCGA | TAATCGTGCCCGGAGTCATG | Start: 1665 |
| Tm | 59.965 | 59.896 | End: 1785 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | AGTCATTCCACTTGCAGCGA | GTAATCGTGCCCGGAGTCAT | Start: 1665 |
| Tm | 59.965 | 59.896 | End: 1786 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | CGGATGAGAACGGTGGAACA | TAATCGTGCCCGGAGTCATG | Start: 1638 |
| Tm | 60.038 | 59.896 | End: 1785 |
| Size | 20 | 20 | 148 |
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