ssr_primer
Primer Details for TRINITY_DN69303_c1_g1_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCTTCCCAGCCACCGTAC | TAGTCCTCCCCCTCGGAAAG | Start: 758 |
| Tm | 59.964 | 60.032 | End: 963 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | ATCGTGAGGCCTGAGTCTCT | CAACCCTCACACCCCAGATC | Start: 907 |
| Tm | 60.034 | 60.035 | End: 1140 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | TTTCTTCCCAGCCACCGTAC | AGAGACTCAGGCCTCACGAT | Start: 758 |
| Tm | 59.964 | 60.034 | End: 926 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | TACCGGCTCCAAATGGTTCC | GTACGGTGGCTGGGAAGAAA | Start: 543 |
| Tm | 60.035 | 59.964 | End: 777 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | CTGCTCACCAGGACTGAAGG | GTACGGTGGCTGGGAAGAAA | Start: 585 |
| Tm | 60.037 | 59.964 | End: 777 |
| Size | 20 | 20 | 193 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCTTCCCAGCCACCGTAC | TAGTCCTCCCCCTCGGAAAG | Start: 758 |
| Tm | 59.964 | 60.032 | End: 963 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | ATCGTGAGGCCTGAGTCTCT | CAACCCTCACACCCCAGATC | Start: 907 |
| Tm | 60.034 | 60.035 | End: 1140 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | TTTCTTCCCAGCCACCGTAC | AGAGACTCAGGCCTCACGAT | Start: 758 |
| Tm | 59.964 | 60.034 | End: 926 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | TACCGGCTCCAAATGGTTCC | GTACGGTGGCTGGGAAGAAA | Start: 543 |
| Tm | 60.035 | 59.964 | End: 777 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | CTGCTCACCAGGACTGAAGG | GTACGGTGGCTGGGAAGAAA | Start: 585 |
| Tm | 60.037 | 59.964 | End: 777 |
| Size | 20 | 20 | 193 |
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