ssr_primer
Primer Details for TRINITY_DN69275_c2_g2_i2 |
|---|
| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCACTTGCTTTGCACAG | AGCAACCAGTAATCGGCACA | Start: 58 |
| Tm | 59.967 | 59.964 | End: 309 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | TGTGCCGATTACTGGTTGCT | GTGAGGTCCGTTTCCTGGAG | Start: 290 |
| Tm | 59.964 | 60.037 | End: 506 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | AATCACCTCAGCGCCTCTTC | Start: 487 |
| Tm | 60.037 | 60.108 | End: 729 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | GCGCCTCTTCCCTGTATTCA | Start: 487 |
| Tm | 60.037 | 59.822 | End: 719 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | CAGCGCCTCTTCCCTGTATT | Start: 487 |
| Tm | 60.037 | 59.821 | End: 721 |
| Size | 20 | 20 | 235 |
(G)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCACTTGCTTTGCACAG | AGCAACCAGTAATCGGCACA | Start: 58 |
| Tm | 59.967 | 59.964 | End: 309 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | TGTGCCGATTACTGGTTGCT | GTGAGGTCCGTTTCCTGGAG | Start: 290 |
| Tm | 59.964 | 60.037 | End: 506 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | AATCACCTCAGCGCCTCTTC | Start: 487 |
| Tm | 60.037 | 60.108 | End: 729 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | GCGCCTCTTCCCTGTATTCA | Start: 487 |
| Tm | 60.037 | 59.822 | End: 719 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | CAGCGCCTCTTCCCTGTATT | Start: 487 |
| Tm | 60.037 | 59.821 | End: 721 |
| Size | 20 | 20 | 235 |
(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCACTTGCTTTGCACAG | AGCAACCAGTAATCGGCACA | Start: 58 |
| Tm | 59.967 | 59.964 | End: 309 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | TGTGCCGATTACTGGTTGCT | GTGAGGTCCGTTTCCTGGAG | Start: 290 |
| Tm | 59.964 | 60.037 | End: 506 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | AATCACCTCAGCGCCTCTTC | Start: 487 |
| Tm | 60.037 | 60.108 | End: 729 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | GCGCCTCTTCCCTGTATTCA | Start: 487 |
| Tm | 60.037 | 59.822 | End: 719 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | CTCCAGGAAACGGACCTCAC | CAGCGCCTCTTCCCTGTATT | Start: 487 |
| Tm | 60.037 | 59.821 | End: 721 |
| Size | 20 | 20 | 235 |
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