Primer Details for TRINITY_DN69251_c5_g1_i1 | |||
|---|---|---|---|
| (A)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTGTAGTAGTTATACGCAACA | GGCGCATAAGTGTACTTTTTGC | Start: 119 |
| Tm | 58.288 | 59.085 | End: 353 |
| Size | 24 | 22 | 235 |
| Primer 2 details | |||
| Sequence | TGCTTGTAGTAGTTATACGCAACA | GGCGCATAAGTGTACTTTTTGCT | Start: 119 |
| Tm | 58.288 | 60.366 | End: 353 |
| Size | 24 | 23 | 235 |
| Primer 3 details | |||
| Sequence | TGCTTGTAGTAGTTATACGCAACA | AGGCGCATAAGTGTACTTTTTGC | Start: 119 |
| Tm | 58.288 | 60.366 | End: 354 |
| Size | 24 | 23 | 236 |
| Primer 4 details | |||
| Sequence | TGCTTGTAGTAGTTATACGCAACAT | GGCGCATAAGTGTACTTTTTGC | Start: 119 |
| Tm | 58.665 | 59.085 | End: 353 |
| Size | 25 | 22 | 235 |
| Primer 5 details | |||
| Sequence | ATGCTTGTAGTAGTTATACGCAACA | GGCGCATAAGTGTACTTTTTGC | Start: 118 |
| Tm | 58.665 | 59.085 | End: 353 |
| Size | 25 | 22 | 236 |
