Primer Details for TRINITY_DN69158_c1_g1_i1 |
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| (T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGAAAAGAGAGCATGTATGTCGT | CTGCCGTGCGAAGGAAGAA | Start: 108 |
| Tm | 59.065 | 60.668 | End: 207 |
| Size | 25 | 19 | 100 |
| Primer 2 details |
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| Sequence | TCAAAGAAAAGAGAGCATGTATGTCG | CTGCCGTGCGAAGGAAGAA | Start: 106 |
| Tm | 59.909 | 60.668 | End: 207 |
| Size | 26 | 19 | 102 |
| Primer 3 details |
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| Sequence | AAAGAAAAGAGAGCATGTATGTCGTC | CTGCCGTGCGAAGGAAGAA | Start: 108 |
| Tm | 59.909 | 60.668 | End: 207 |
| Size | 26 | 19 | 100 |
| Primer 4 details |
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| Sequence | CAAAGAAAAGAGAGCATGTATGTCGT | CTGCCGTGCGAAGGAAGAA | Start: 107 |
| Tm | 60.128 | 60.668 | End: 207 |
| Size | 26 | 19 | 101 |
| Primer 5 details |
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| Sequence | AAAGAAAAGAGAGCATGTATGTCGT | CTGCCGTGCGAAGGAAGAAC | Start: 108 |
| Tm | 59.065 | 61.902 | End: 207 |
| Size | 25 | 20 | 100 |
