ssr_primer
Primer Details for TRINITY_DN69138_c5_g3_i1 |
|---|
| (C)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGACGCTGTTGCATTAGC | ACAGGGTTTGCTGGACAACA | Start: 51 |
| Tm | 59.902 | 60.034 | End: 215 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | CCTGACGCTGTTGCATTAGC | CTGCTACAGGGTTTGCTGGA | Start: 51 |
| Tm | 59.902 | 59.963 | End: 220 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
|---|
| Sequence | CCTGACGCTGTTGCATTAGC | TCTGCTACAGGGTTTGCTGG | Start: 51 |
| Tm | 59.902 | 59.963 | End: 221 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | GCCCATTCCAGCCTACCATT | ACAGGGTTTGCTGGACAACA | Start: 116 |
| Tm | 60.106 | 60.034 | End: 215 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | AATGCCCATTCCAGCCTACC | ACAGGGTTTGCTGGACAACA | Start: 113 |
| Tm | 60.106 | 60.034 | End: 215 |
| Size | 20 | 20 | 103 |
(T)10ctttttaaattgtacagaattagtgacttacatgatcagtcctgctgattttttctttc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGACGCTGTTGCATTAGC | ACAGGGTTTGCTGGACAACA | Start: 51 |
| Tm | 59.902 | 60.034 | End: 215 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | CCTGACGCTGTTGCATTAGC | CTGCTACAGGGTTTGCTGGA | Start: 51 |
| Tm | 59.902 | 59.963 | End: 220 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
|---|
| Sequence | CCTGACGCTGTTGCATTAGC | TCTGCTACAGGGTTTGCTGG | Start: 51 |
| Tm | 59.902 | 59.963 | End: 221 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | GCCCATTCCAGCCTACCATT | ACAGGGTTTGCTGGACAACA | Start: 116 |
| Tm | 60.106 | 60.034 | End: 215 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | AATGCCCATTCCAGCCTACC | ACAGGGTTTGCTGGACAACA | Start: 113 |
| Tm | 60.106 | 60.034 | End: 215 |
| Size | 20 | 20 | 103 |
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