ssr_primer
Primer Details for TRINITY_DN69105_c1_g1_i2 |
|---|
| (A)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTTGCGCCTACATTCTGG | CGACCCACGTTGCCAATTTT | Start: 172 |
| Tm | 59.903 | 59.969 | End: 352 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | AAAATTGGCAACGTGGGTCG | ATTGCAGTGCACACCTCTCA | Start: 333 |
| Tm | 59.969 | 59.891 | End: 450 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
|---|
| Sequence | AGGATTGGCCGTTGAGTCTG | GCCAGGGGTCTACCGTTATG | Start: 2224 |
| Tm | 60.036 | 59.894 | End: 2374 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | CAGGATTGGCCGTTGAGTCT | GCCAGGGGTCTACCGTTATG | Start: 2223 |
| Tm | 60.036 | 59.894 | End: 2374 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | TACGATGTGAGCACCAGCAG | AGACTCAACGGCCAATCCTG | Start: 2029 |
| Tm | 60.109 | 60.036 | End: 2242 |
| Size | 20 | 20 | 214 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTTGCGCCTACATTCTGG | CGACCCACGTTGCCAATTTT | Start: 172 |
| Tm | 59.903 | 59.969 | End: 352 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | AAAATTGGCAACGTGGGTCG | ATTGCAGTGCACACCTCTCA | Start: 333 |
| Tm | 59.969 | 59.891 | End: 450 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
|---|
| Sequence | AGGATTGGCCGTTGAGTCTG | GCCAGGGGTCTACCGTTATG | Start: 2224 |
| Tm | 60.036 | 59.894 | End: 2374 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | CAGGATTGGCCGTTGAGTCT | GCCAGGGGTCTACCGTTATG | Start: 2223 |
| Tm | 60.036 | 59.894 | End: 2374 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | TACGATGTGAGCACCAGCAG | AGACTCAACGGCCAATCCTG | Start: 2029 |
| Tm | 60.109 | 60.036 | End: 2242 |
| Size | 20 | 20 | 214 |
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