ssr_primer
Primer Details for TRINITY_DN69100_c2_g5_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGCAGAACACAGCATCAT | GGTGGTGAACCCCCATGAAA | Start: 497 |
| Tm | 60.036 | 60.179 | End: 636 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
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| Sequence | CTGCTGGGATCTCAGGCTTT | ATGATGCTGTGTTCTGCCGA | Start: 281 |
| Tm | 59.745 | 60.036 | End: 516 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
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| Sequence | GCAGTTTGAAACAGTCGGCA | AGACAGCGCAGACAAGGTAC | Start: 483 |
| Tm | 59.62 | 60.038 | End: 592 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | GCAGTTTGAAACAGTCGGCA | CGCAGACAAGGTACGTTCCT | Start: 483 |
| Tm | 59.62 | 60.039 | End: 586 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
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| Sequence | GTACCTTGTCTGCGCTGTCT | TCTCCCGCACAAAGAAGTGT | Start: 573 |
| Tm | 60.038 | 59.532 | End: 793 |
| Size | 20 | 20 | 221 |
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