ssr_primer
Primer Details for TRINITY_DN69074_c4_g7_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGGGGTTTTTGGGTGCTG | AGTTCGCCGTTAGCCGATAG | Start: 130 |
| Tm | 59.893 | 59.97 | End: 281 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
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| Sequence | CACTTGGGGTTTTTGGGTGC | AGTTCGCCGTTAGCCGATAG | Start: 128 |
| Tm | 60.179 | 59.97 | End: 281 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
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| Sequence | CTTGGGGTTTTTGGGTGCTG | TAGAAGTTCGCCGTTAGCCG | Start: 130 |
| Tm | 59.893 | 60.179 | End: 285 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
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| Sequence | CTTGGGGTTTTTGGGTGCTG | ACGGCCTCGACATCTCTAGA | Start: 130 |
| Tm | 59.893 | 59.819 | End: 301 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
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| Sequence | TGGGGTTTTTGGGTGCTGTA | AGTTCGCCGTTAGCCGATAG | Start: 132 |
| Tm | 59.738 | 59.97 | End: 281 |
| Size | 20 | 20 | 150 |
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