ssr_primer
Primer Details for TRINITY_DN69050_c0_g1_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCACTATGCGTCGCCAT | ATTTGCTGCAATTCACGGGT | Start: 85 |
| Tm | 60.108 | 59.032 | End: 331 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | ACCCTGTCGCATTGCTGAAT | ATTTGCTGCAATTCACGGGT | Start: 113 |
| Tm | 60.323 | 59.032 | End: 331 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | CCATGAGGAGCAACCCTGTC | ATTTGCTGCAATTCACGGGT | Start: 101 |
| Tm | 60.394 | 59.032 | End: 331 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | TCCTTTCCACTATGCGTCGC | ATTTGCTGCAATTCACGGGT | Start: 82 |
| Tm | 60.46 | 59.032 | End: 331 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | TTTCCACTATGCGTCGCCAT | AATTTGCTGCAATTCACGGGT | Start: 85 |
| Tm | 60.108 | 59.656 | End: 332 |
| Size | 20 | 21 | 248 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCACTATGCGTCGCCAT | ATTTGCTGCAATTCACGGGT | Start: 85 |
| Tm | 60.108 | 59.032 | End: 331 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | ACCCTGTCGCATTGCTGAAT | ATTTGCTGCAATTCACGGGT | Start: 113 |
| Tm | 60.323 | 59.032 | End: 331 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | CCATGAGGAGCAACCCTGTC | ATTTGCTGCAATTCACGGGT | Start: 101 |
| Tm | 60.394 | 59.032 | End: 331 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | TCCTTTCCACTATGCGTCGC | ATTTGCTGCAATTCACGGGT | Start: 82 |
| Tm | 60.46 | 59.032 | End: 331 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | TTTCCACTATGCGTCGCCAT | AATTTGCTGCAATTCACGGGT | Start: 85 |
| Tm | 60.108 | 59.656 | End: 332 |
| Size | 20 | 21 | 248 |
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