ssr_primer
Primer Details for TRINITY_DN68896_c1_g2_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCCGACACTTTCTCAGAT | GGAGAGTCCATCTGCACACC | Start: 348 |
| Tm | 60.109 | 60.108 | End: 450 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
|---|
| Sequence | CCGCCGACACTTTCTCAGAT | GAGTCCATCTGCACACCGAT | Start: 348 |
| Tm | 60.109 | 59.823 | End: 447 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | CGTGACACGCGGTACCTTTA | ATCTGAGAAAGTGTCGGCGG | Start: 109 |
| Tm | 60.387 | 60.109 | End: 367 |
| Size | 20 | 20 | 259 |
| Primer 4 details |
|---|
| Sequence | CCGCCGACACTTTCTCAGAT | GAGAGTCCATCTGCACACCG | Start: 348 |
| Tm | 60.109 | 60.459 | End: 449 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | TGCGGAAATTGTTTGGTGCA | GGAGAGTCCATCTGCACACC | Start: 327 |
| Tm | 59.538 | 60.108 | End: 450 |
| Size | 20 | 20 | 124 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCCGACACTTTCTCAGAT | GGAGAGTCCATCTGCACACC | Start: 348 |
| Tm | 60.109 | 60.108 | End: 450 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
|---|
| Sequence | CCGCCGACACTTTCTCAGAT | GAGTCCATCTGCACACCGAT | Start: 348 |
| Tm | 60.109 | 59.823 | End: 447 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | CGTGACACGCGGTACCTTTA | ATCTGAGAAAGTGTCGGCGG | Start: 109 |
| Tm | 60.387 | 60.109 | End: 367 |
| Size | 20 | 20 | 259 |
| Primer 4 details |
|---|
| Sequence | CCGCCGACACTTTCTCAGAT | GAGAGTCCATCTGCACACCG | Start: 348 |
| Tm | 60.109 | 60.459 | End: 449 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | TGCGGAAATTGTTTGGTGCA | GGAGAGTCCATCTGCACACC | Start: 327 |
| Tm | 59.538 | 60.108 | End: 450 |
| Size | 20 | 20 | 124 |
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