ssr_primer
Primer Details for TRINITY_DN68889_c1_g1_i4 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACGAGAACAAAGAGGCC | CGCGAACCTTGCTTCACAAA | Start: 538 |
| Tm | 60.039 | 59.971 | End: 802 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | CAAGCGACTCCGTCACTCTT | GATCCGAGGGTAGTGCGATG | Start: 28 |
| Tm | 60.039 | 60.039 | End: 129 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | CGAAGATGAGGATCCCACGG | GTCTTTCGCAGACCAATCGC | Start: 1271 |
| Tm | 59.967 | 59.905 | End: 1388 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
|---|
| Sequence | TGCGGGATCTCGACAATGAG | GCTGTTTGCTGAAACGCTGA | Start: 1681 |
| Tm | 59.897 | 59.971 | End: 1829 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | CTGCGGGATCTCGACAATGA | GCTGTTTGCTGAAACGCTGA | Start: 1680 |
| Tm | 59.897 | 59.971 | End: 1829 |
| Size | 20 | 20 | 150 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACGAGAACAAAGAGGCC | CGCGAACCTTGCTTCACAAA | Start: 538 |
| Tm | 60.039 | 59.971 | End: 802 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | CAAGCGACTCCGTCACTCTT | GATCCGAGGGTAGTGCGATG | Start: 28 |
| Tm | 60.039 | 60.039 | End: 129 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | CGAAGATGAGGATCCCACGG | GTCTTTCGCAGACCAATCGC | Start: 1271 |
| Tm | 59.967 | 59.905 | End: 1388 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
|---|
| Sequence | TGCGGGATCTCGACAATGAG | GCTGTTTGCTGAAACGCTGA | Start: 1681 |
| Tm | 59.897 | 59.971 | End: 1829 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | CTGCGGGATCTCGACAATGA | GCTGTTTGCTGAAACGCTGA | Start: 1680 |
| Tm | 59.897 | 59.971 | End: 1829 |
| Size | 20 | 20 | 150 |
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