ssr_primer
Primer Details for TRINITY_DN68876_c3_g2_i5 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAATAGCGGCAACACTCG | GTGGTAAAAGGGAGGGGGTG | Start: 64 |
| Tm | 59.975 | 59.961 | End: 172 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | CGAAATAGCGGCAACACTCG | TGACGACAAGACGGACGTTT | Start: 64 |
| Tm | 59.975 | 59.899 | End: 281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CGAGGAAGCTTCTGTGCAGA | TGACGACAAGACGGACGTTT | Start: 95 |
| Tm | 60.038 | 59.899 | End: 281 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | TCGAGGAAGCTTCTGTGCAG | TGACGACAAGACGGACGTTT | Start: 94 |
| Tm | 60.038 | 59.899 | End: 281 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
|---|
| Sequence | CACCCCCTCCCTTTTACCAC | TGACGACAAGACGGACGTTT | Start: 153 |
| Tm | 59.961 | 59.899 | End: 281 |
| Size | 20 | 20 | 129 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAATAGCGGCAACACTCG | GTGGTAAAAGGGAGGGGGTG | Start: 64 |
| Tm | 59.975 | 59.961 | End: 172 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | CGAAATAGCGGCAACACTCG | TGACGACAAGACGGACGTTT | Start: 64 |
| Tm | 59.975 | 59.899 | End: 281 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CGAGGAAGCTTCTGTGCAGA | TGACGACAAGACGGACGTTT | Start: 95 |
| Tm | 60.038 | 59.899 | End: 281 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | TCGAGGAAGCTTCTGTGCAG | TGACGACAAGACGGACGTTT | Start: 94 |
| Tm | 60.038 | 59.899 | End: 281 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
|---|
| Sequence | CACCCCCTCCCTTTTACCAC | TGACGACAAGACGGACGTTT | Start: 153 |
| Tm | 59.961 | 59.899 | End: 281 |
| Size | 20 | 20 | 129 |
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