ssr_primer
Primer Details for TRINITY_DN68830_c5_g4_i1 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTTACCGGCCCTCAAAAT | GTCCAATGGGTCGGCAAAAG | Start: 19 |
| Tm | 59.963 | 59.756 | End: 291 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | TTACAACGTTACCGGCCCTC | GTCCAATGGGTCGGCAAAAG | Start: 14 |
| Tm | 60.037 | 59.756 | End: 291 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
|---|
| Sequence | ACGTTACCGGCCCTCAAAAT | CAATGGGTCGGCAAAAGCAT | Start: 19 |
| Tm | 59.963 | 59.753 | End: 288 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | TTACAACGTTACCGGCCCTC | CAATGGGTCGGCAAAAGCAT | Start: 14 |
| Tm | 60.037 | 59.753 | End: 288 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | AACGTTACCGGCCCTCAAAA | GTCCAATGGGTCGGCAAAAG | Start: 18 |
| Tm | 60.179 | 59.756 | End: 291 |
| Size | 20 | 20 | 274 |
(C)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTTACCGGCCCTCAAAAT | GTCCAATGGGTCGGCAAAAG | Start: 19 |
| Tm | 59.963 | 59.756 | End: 291 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | TTACAACGTTACCGGCCCTC | GTCCAATGGGTCGGCAAAAG | Start: 14 |
| Tm | 60.037 | 59.756 | End: 291 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
|---|
| Sequence | ACGTTACCGGCCCTCAAAAT | CAATGGGTCGGCAAAAGCAT | Start: 19 |
| Tm | 59.963 | 59.753 | End: 288 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | TTACAACGTTACCGGCCCTC | CAATGGGTCGGCAAAAGCAT | Start: 14 |
| Tm | 60.037 | 59.753 | End: 288 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | AACGTTACCGGCCCTCAAAA | GTCCAATGGGTCGGCAAAAG | Start: 18 |
| Tm | 60.179 | 59.756 | End: 291 |
| Size | 20 | 20 | 274 |
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