ssr_primer
Primer Details for TRINITY_DN68781_c4_g1_i7 |
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| (T)24caaattttctgggggttttaaatatttaattctcaccaaag(A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTCGATGCCAACCGAGA | GAGAATGTTGACGGAGGCCA | Start: 685 |
| Tm | 59.966 | 60.036 | End: 813 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | TTGTTCGATGCCAACCGAGA | TGAGAATGTTGACGGAGGCC | Start: 685 |
| Tm | 59.966 | 60.036 | End: 814 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | ACGATTGGAGCAGGAAGTGG | ATTTGGTCGCTCTGAGGGTG | Start: 1464 |
| Tm | 60.036 | 60.036 | End: 1565 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
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| Sequence | ACGATTGGAGCAGGAAGTGG | TTGCTCTCCTTTCTCCTGCG | Start: 1464 |
| Tm | 60.036 | 60.037 | End: 1636 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
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| Sequence | GCTGAACTCATCGGACCGAA | TCTCGGTTGGCATCGAACAA | Start: 601 |
| Tm | 60.109 | 59.966 | End: 704 |
| Size | 20 | 20 | 104 |
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