ssr_primer
Primer Details for TRINITY_DN68691_c3_g1_i1 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAGCTCGGCCAATTCTGT | TTGAGCGCTGCAAAACTGTC | Start: 772 |
| Tm | 59.963 | 59.971 | End: 1004 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | GACAGTTTTGCAGCGCTCAA | GGTTGCCAAATTGGTCGGTC | Start: 985 |
| Tm | 59.971 | 60.039 | End: 1125 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | AGTTACTGTCAGTGGCGTGG | ATTAGGGGTCGCCAACAGTG | Start: 281 |
| Tm | 59.966 | 60.035 | End: 468 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | ACAGAACCCGCGGAAAATCT | GGCAGATGCGCAAAAGAGAG | Start: 1393 |
| Tm | 59.964 | 59.902 | End: 1496 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | ACAGAACCCGCGGAAAATCT | AGAGACAACTCGGCAGATGC | Start: 1393 |
| Tm | 59.964 | 60.108 | End: 1507 |
| Size | 20 | 20 | 115 |
(A)17cgattgtgtaaaagaaatttt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAGCTCGGCCAATTCTGT | TTGAGCGCTGCAAAACTGTC | Start: 772 |
| Tm | 59.963 | 59.971 | End: 1004 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | GACAGTTTTGCAGCGCTCAA | GGTTGCCAAATTGGTCGGTC | Start: 985 |
| Tm | 59.971 | 60.039 | End: 1125 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | AGTTACTGTCAGTGGCGTGG | ATTAGGGGTCGCCAACAGTG | Start: 281 |
| Tm | 59.966 | 60.035 | End: 468 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | ACAGAACCCGCGGAAAATCT | GGCAGATGCGCAAAAGAGAG | Start: 1393 |
| Tm | 59.964 | 59.902 | End: 1496 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | ACAGAACCCGCGGAAAATCT | AGAGACAACTCGGCAGATGC | Start: 1393 |
| Tm | 59.964 | 60.108 | End: 1507 |
| Size | 20 | 20 | 115 |
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