ssr_primer
Primer Details for TRINITY_DN68630_c4_g2_i2 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCTAGGATAATCTGCGCA | TGGATTGAGCCTTTCGACCC | Start: 432 |
| Tm | 60.038 | 60.035 | End: 705 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
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| Sequence | TGCGGCTAGGATAATCTGCG | TGGATTGAGCCTTTCGACCC | Start: 430 |
| Tm | 60.038 | 60.035 | End: 705 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
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| Sequence | TGCTGCCGTTGTTGTTTCTG | GCTCCTCAAACGAGACACCA | Start: 541 |
| Tm | 59.9 | 59.966 | End: 780 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
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| Sequence | GCGACATGTTGCCCAATACC | GCTCCTCAAACGAGACACCA | Start: 609 |
| Tm | 59.899 | 59.966 | End: 780 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
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| Sequence | GCTGCCGTTGTTGTTTCTGT | GCTCCTCAAACGAGACACCA | Start: 542 |
| Tm | 59.899 | 59.966 | End: 780 |
| Size | 20 | 20 | 239 |
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