ssr_primer
Primer Details for TRINITY_DN68614_c2_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAGATGCTCAGAGGGTTT | GCGCACAGTGTCAAAATCTGT | Start: 317 |
| Tm | 60.035 | 60.002 | End: 564 |
| Size | 20 | 21 | 248 |
| Primer 2 details |
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| Sequence | GCCAGATGCTCAGAGGGTTT | TGCGCACAGTGTCAAAATCTG | Start: 317 |
| Tm | 60.035 | 60.003 | End: 565 |
| Size | 20 | 21 | 249 |
| Primer 3 details |
|---|
| Sequence | GCCAGATGCTCAGAGGGTTT | TGCGCACAGTGTCAAAATCT | Start: 317 |
| Tm | 60.035 | 58.692 | End: 565 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
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| Sequence | ACAGATTTTGACACTGTGCGC | AACCCTGTAACAGCACTGGC | Start: 544 |
| Tm | 60.002 | 60.537 | End: 650 |
| Size | 21 | 20 | 107 |
| Primer 5 details |
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| Sequence | CAGATTTTGACACTGTGCGCA | AACCCTGTAACAGCACTGGC | Start: 545 |
| Tm | 60.003 | 60.537 | End: 650 |
| Size | 21 | 20 | 106 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAGATGCTCAGAGGGTTT | GCGCACAGTGTCAAAATCTGT | Start: 317 |
| Tm | 60.035 | 60.002 | End: 564 |
| Size | 20 | 21 | 248 |
| Primer 2 details |
|---|
| Sequence | GCCAGATGCTCAGAGGGTTT | TGCGCACAGTGTCAAAATCTG | Start: 317 |
| Tm | 60.035 | 60.003 | End: 565 |
| Size | 20 | 21 | 249 |
| Primer 3 details |
|---|
| Sequence | GCCAGATGCTCAGAGGGTTT | TGCGCACAGTGTCAAAATCT | Start: 317 |
| Tm | 60.035 | 58.692 | End: 565 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
|---|
| Sequence | ACAGATTTTGACACTGTGCGC | AACCCTGTAACAGCACTGGC | Start: 544 |
| Tm | 60.002 | 60.537 | End: 650 |
| Size | 21 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | CAGATTTTGACACTGTGCGCA | AACCCTGTAACAGCACTGGC | Start: 545 |
| Tm | 60.003 | 60.537 | End: 650 |
| Size | 21 | 20 | 106 |
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