ssr_primer
Primer Details for TRINITY_DN68545_c3_g2_i6 |
|---|
| (T)12aattgaaaaaagttttttaacggtcggtcattgttgctggccagggaataaattttttttgtcaaaaagtcagaatgaagtagcagacgggga(T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTGAGGGAGGAGAGGAG | TAACGCCAGTCACGAGCTTT | Start: 206 |
| Tm | 59.96 | 59.967 | End: 383 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GTGGGTTTGGGGTACGGAAT | AACAACCCGATTCCATGGCT | Start: 73 |
| Tm | 59.961 | 59.96 | End: 332 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | GTGGGTTTGGGGTACGGAAT | CTCCTCTCCTCCCTCACACA | Start: 73 |
| Tm | 59.961 | 59.96 | End: 225 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | TGTGTGAGGGAGGAGAGGAG | AACAACCCGATTCCATGGCT | Start: 206 |
| Tm | 59.96 | 59.96 | End: 332 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TGTGTGAGGGAGGAGAGGAG | CACATTAGGAGTTGCTGCGC | Start: 206 |
| Tm | 59.96 | 59.902 | End: 354 |
| Size | 20 | 20 | 149 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTGAGGGAGGAGAGGAG | TAACGCCAGTCACGAGCTTT | Start: 206 |
| Tm | 59.96 | 59.967 | End: 383 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GTGGGTTTGGGGTACGGAAT | AACAACCCGATTCCATGGCT | Start: 73 |
| Tm | 59.961 | 59.96 | End: 332 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | GTGGGTTTGGGGTACGGAAT | CTCCTCTCCTCCCTCACACA | Start: 73 |
| Tm | 59.961 | 59.96 | End: 225 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | TGTGTGAGGGAGGAGAGGAG | AACAACCCGATTCCATGGCT | Start: 206 |
| Tm | 59.96 | 59.96 | End: 332 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TGTGTGAGGGAGGAGAGGAG | CACATTAGGAGTTGCTGCGC | Start: 206 |
| Tm | 59.96 | 59.902 | End: 354 |
| Size | 20 | 20 | 149 |
(T)16gaaagaactgttttacactgctgtgt(C)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTGAGGGAGGAGAGGAG | TAACGCCAGTCACGAGCTTT | Start: 206 |
| Tm | 59.96 | 59.967 | End: 383 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GTGGGTTTGGGGTACGGAAT | AACAACCCGATTCCATGGCT | Start: 73 |
| Tm | 59.961 | 59.96 | End: 332 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | GTGGGTTTGGGGTACGGAAT | CTCCTCTCCTCCCTCACACA | Start: 73 |
| Tm | 59.961 | 59.96 | End: 225 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | TGTGTGAGGGAGGAGAGGAG | AACAACCCGATTCCATGGCT | Start: 206 |
| Tm | 59.96 | 59.96 | End: 332 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TGTGTGAGGGAGGAGAGGAG | CACATTAGGAGTTGCTGCGC | Start: 206 |
| Tm | 59.96 | 59.902 | End: 354 |
| Size | 20 | 20 | 149 |
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