ssr_primer
Primer Details for TRINITY_DN68535_c2_g5_i1 |
|---|
| (A)27: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCAATAAGATCGGGACCG | AAAATCTGCGAATGCCGAGC | Start: 274 |
| Tm | 60.249 | 59.901 | End: 515 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | GGGACCGCAAAGACTGAACT | AAAATCTGCGAATGCCGAGC | Start: 287 |
| Tm | 60.25 | 59.901 | End: 515 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GGGACCGCAAAGACTGAACT | CGACTGATGGAATGGCAGGA | Start: 287 |
| Tm | 60.25 | 59.821 | End: 555 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | GGGACCGCAAAGACTGAACT | TCGACTGATGGAATGGCAGG | Start: 287 |
| Tm | 60.25 | 59.821 | End: 556 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
|---|
| Sequence | AAGATCGGGACCGCAAAGAC | AAAATCTGCGAATGCCGAGC | Start: 281 |
| Tm | 60.391 | 59.901 | End: 515 |
| Size | 20 | 20 | 235 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCAATAAGATCGGGACCG | AAAATCTGCGAATGCCGAGC | Start: 274 |
| Tm | 60.249 | 59.901 | End: 515 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | GGGACCGCAAAGACTGAACT | AAAATCTGCGAATGCCGAGC | Start: 287 |
| Tm | 60.25 | 59.901 | End: 515 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GGGACCGCAAAGACTGAACT | CGACTGATGGAATGGCAGGA | Start: 287 |
| Tm | 60.25 | 59.821 | End: 555 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | GGGACCGCAAAGACTGAACT | TCGACTGATGGAATGGCAGG | Start: 287 |
| Tm | 60.25 | 59.821 | End: 556 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
|---|
| Sequence | AAGATCGGGACCGCAAAGAC | AAAATCTGCGAATGCCGAGC | Start: 281 |
| Tm | 60.391 | 59.901 | End: 515 |
| Size | 20 | 20 | 235 |
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