ssr_primer
Primer Details for TRINITY_DN68478_c1_g1_i2 |
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| (G)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGCATTAGTTTTCCGGAC | GGCCGAGTTTCATGCGAATC | Start: 16 |
| Tm | 59.902 | 59.972 | End: 263 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
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| Sequence | CTGGCGGCATTAGTTTTCCG | GGCCGAGTTTCATGCGAATC | Start: 13 |
| Tm | 59.901 | 59.972 | End: 263 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
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| Sequence | GATTCGCATGAAACTCGGCC | CATTGTTGATGCGCGGTCAA | Start: 244 |
| Tm | 59.972 | 60.11 | End: 361 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
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| Sequence | AATTCGACCATGACGGGACC | CGGGGTAAGGAAGGGGAGTA | Start: 1498 |
| Tm | 60.108 | 60.032 | End: 1641 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
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| Sequence | AAAGCTGTTCCTTTGGGGCT | TTCTCGTGACTTGCTGCCAT | Start: 1016 |
| Tm | 60.106 | 59.965 | End: 1185 |
| Size | 20 | 20 | 170 |
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