ssr_primer
Primer Details for TRINITY_DN68422_c5_g6_i1 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATGAGCCCGTTGCAAAC | AAATGTTCACCTGTGCGTGC | Start: 376 |
| Tm | 59.968 | 59.969 | End: 558 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | GCACGCACAGGTGAACATTT | AAATCGTGAGCTCTTCGCCA | Start: 539 |
| Tm | 59.969 | 60.037 | End: 776 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
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| Sequence | GGTTCCTGGCGGTCCTAAAA | GTTTGCAACGGGCTCATTGT | Start: 136 |
| Tm | 59.963 | 59.968 | End: 395 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
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| Sequence | AGAGAATTTGCGGCGACTGA | GTTTGCAACGGGCTCATTGT | Start: 169 |
| Tm | 60.037 | 59.968 | End: 395 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
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| Sequence | TCAGAGAATTTGCGGCGACT | GTTTGCAACGGGCTCATTGT | Start: 167 |
| Tm | 60.037 | 59.968 | End: 395 |
| Size | 20 | 20 | 229 |
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