ssr_primer
Primer Details for TRINITY_DN68356_c1_g2_i2 |
|---|
| (T)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGTGCGATTGGCTACAGC | GTTTCTGCTGCACCTTGCTC | Start: 560 |
| Tm | 59.902 | 60.04 | End: 707 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | GACCCAGATGACGACAGCAA | CAGACTTCGCTCTGGAGGTG | Start: 613 |
| Tm | 60.037 | 60.109 | End: 821 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | CAAGTGCGATTGGCTACAGC | CAGACTTCGCTCTGGAGGTG | Start: 560 |
| Tm | 59.902 | 60.109 | End: 821 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | CAAGTGCGATTGGCTACAGC | TCTTCGGTTACTTCGACGGC | Start: 560 |
| Tm | 59.902 | 60.11 | End: 671 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | GACCCAGATGACGACAGCAA | GACAGACTTCGCTCTGGAGG | Start: 613 |
| Tm | 60.037 | 59.828 | End: 823 |
| Size | 20 | 20 | 211 |
(T)10atttttttctccttttcttttttctttttttttctttttttagacattttagtgttaaaaaacaacagcagagtttaagtatttcaagag(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGTGCGATTGGCTACAGC | GTTTCTGCTGCACCTTGCTC | Start: 560 |
| Tm | 59.902 | 60.04 | End: 707 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | GACCCAGATGACGACAGCAA | CAGACTTCGCTCTGGAGGTG | Start: 613 |
| Tm | 60.037 | 60.109 | End: 821 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | CAAGTGCGATTGGCTACAGC | CAGACTTCGCTCTGGAGGTG | Start: 560 |
| Tm | 59.902 | 60.109 | End: 821 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | CAAGTGCGATTGGCTACAGC | TCTTCGGTTACTTCGACGGC | Start: 560 |
| Tm | 59.902 | 60.11 | End: 671 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | GACCCAGATGACGACAGCAA | GACAGACTTCGCTCTGGAGG | Start: 613 |
| Tm | 60.037 | 59.828 | End: 823 |
| Size | 20 | 20 | 211 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGTGCGATTGGCTACAGC | GTTTCTGCTGCACCTTGCTC | Start: 560 |
| Tm | 59.902 | 60.04 | End: 707 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | GACCCAGATGACGACAGCAA | CAGACTTCGCTCTGGAGGTG | Start: 613 |
| Tm | 60.037 | 60.109 | End: 821 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | CAAGTGCGATTGGCTACAGC | CAGACTTCGCTCTGGAGGTG | Start: 560 |
| Tm | 59.902 | 60.109 | End: 821 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | CAAGTGCGATTGGCTACAGC | TCTTCGGTTACTTCGACGGC | Start: 560 |
| Tm | 59.902 | 60.11 | End: 671 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | GACCCAGATGACGACAGCAA | GACAGACTTCGCTCTGGAGG | Start: 613 |
| Tm | 60.037 | 59.828 | End: 823 |
| Size | 20 | 20 | 211 |
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