ssr_primer
Primer Details for TRINITY_DN68300_c1_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGACTAAAGCGGGCACTCC | GCTCGTCGTGGACTTCTCAT | Start: 537 |
| Tm | 60.036 | 59.829 | End: 671 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
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| Sequence | CGGTACATGGACGAGAGCTC | TTCAACCTCCTCGACCTGGA | Start: 276 |
| Tm | 59.97 | 60.179 | End: 530 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
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| Sequence | GGTCTCCAACACCAACAGCT | GAGCTCTCGTCCATGTACCG | Start: 146 |
| Tm | 60.179 | 59.97 | End: 295 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
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| Sequence | GGTCTCCAACACCAACAGCT | CGAGCTCTCGTCCATGTACC | Start: 146 |
| Tm | 60.179 | 59.97 | End: 296 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
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| Sequence | AAGACTAAAGCGGGCACTCC | TTCAACTTCTTGCCCAGCGA | Start: 537 |
| Tm | 60.036 | 60.179 | End: 748 |
| Size | 20 | 20 | 212 |
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