ssr_primer
Primer Details for TRINITY_DN68291_c5_g2_i2 |
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| (A)11ccccgctgcttccatataacatcgaaggtggtgaaagtagcatgaatgagtcgctttccgcaaccatcttctccctagaaaaaaagaaaaggat(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAACCCCGCTGCTTCCATA | CCCTTCCCCCGGTTCATTAG | Start: 293 |
| Tm | 59.961 | 59.817 | End: 434 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
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| Sequence | CGAGGGAACGGGTCTTTTGT | TATGGAAGCAGCGGGGTTTT | Start: 68 |
| Tm | 60.25 | 59.961 | End: 312 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
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| Sequence | AAACCCCGCTGCTTCCATAT | CCCTTCCCCCGGTTCATTAG | Start: 294 |
| Tm | 59.741 | 59.817 | End: 434 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
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| Sequence | CGAGGGAACGGGTCTTTTGT | ATATGGAAGCAGCGGGGTTT | Start: 68 |
| Tm | 60.25 | 59.741 | End: 313 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
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| Sequence | AGGGAACGGGTCTTTTGTGA | TATGGAAGCAGCGGGGTTTT | Start: 70 |
| Tm | 59.159 | 59.961 | End: 312 |
| Size | 20 | 20 | 243 |
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