ssr_primer
Primer Details for TRINITY_DN68279_c1_g1_i5 |
|---|
| (T)14(G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAACTTCGTCGCTCCGAGT | GCCGCCGTTTGATAGACCTA | Start: 2837 |
| Tm | 59.968 | 59.896 | End: 3114 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | TGGTCGCGGGAAAAAGAGAA | GCTTGTTGTCCAGCCAGTTG | Start: 3036 |
| Tm | 59.893 | 59.969 | End: 3168 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | AAAACTTCGTCGCTCCGAGT | TTCTCTTTTTCCCGCGACCA | Start: 2837 |
| Tm | 59.968 | 59.893 | End: 3055 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | CGCGGGAAAAAGAGAACACG | GCTTGTTGTCCAGCCAGTTG | Start: 3040 |
| Tm | 60.111 | 59.969 | End: 3168 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
|---|
| Sequence | AAAACTTCGTCGCTCCGAGT | CGTGTTCTCTTTTTCCCGCG | Start: 2837 |
| Tm | 59.968 | 60.111 | End: 3059 |
| Size | 20 | 20 | 223 |
(CTTTT)4(CTTTTTTTAAAGGACTAGTGATAGAGTTTGAGATGTCTTGAAAAATTCTTGAATATCCTTGATTTTTTTGAAAATCACCCAAAAAATTTGTGTGTCCTTGAATTTTACATCTGGTTATGTTGTATTTGATATATCAGAAGACCAGGCCTTTTCCTATTTTGCCATGGAGACTTGTATCAAATAGCGCAGCTCATATGTCACATATTTCCTTCTTCAGTAACAAAGAACCAAGTAAGTTAGCAGGAAACCGACATTCTTGAAACATTCATTACGGCTTTTGAAATTTTTTTGGAATGTCCTTAAAAGCCCTTTGAAAATTGTGTTGAAGTTCAGGATACATTAAGTTCTCAGCTGTCCCATTTTTAAAACTGGCCTTCTCACTTTTAAACCTCTTTTCGCCCAAAATTTTTAAAGTTCTTAGTTTCCTCTCGGTAGTTACTCCTGAAAATTTCAGACATTGTCATTGAATTCAGCAGATTTCAGTGCTGCCAGTCGCTGTTACTTAATTTGAGAAACCTACGCGTATCATGAGTACCTTTCTTCTTTTTCGTTTCAGGTGATACAGCCTCTACAAACAGTAGGAAGGTAACCAATGAGCAAGTAGACGATGGTGTAACGTGGACGTATGCTAACCTGTCTGCTACAACCCAAGAACGAATTAAAAGGTTTGAGCAAGAAACCCGTGCTATGCTACAAAGAGACAGGCTCAGAACGGAAAACGAGAAGGAGAGACTGGAGAGGGAGTGGCAACAAGCTAAACGGGAACTGGAAGCTGATGATGCTTTAGATCGGCTAGCGGATGATCCCTCAGCACTACATGAACTATCGGAAACAACATTGTTAAACAAAGAATCATCATCTGAAGATTTAAGTCCGACATTAAACAATAAAGTGTCCAACATGAAAAGGTTCAAAACAGGAAACGAAGTCTCTACAATTTCCAATGGAAAATCTAGTGGCTCTCTTGACTCTTTGAAAGAATCTAGTGTTACAATGGGAAGGACACCCAGTAATGCTTCCGATGAAGGAAGTGATTTATTCACAAGCCTTACATCTACTTTTGACAAGAAATTGAAATCTTTACTAAAATCTGAGGAACTTTGTAATGATGACGTAGAAGAGCATGTCAGTACAATGCACGATGAGAAAGTATCAACAAAATTTCAAGATCCCAGCCTGCATAGATCCTTGGAAAAGCAGTACAAGCAGGTATCAAAAAGTGAGAAAGATGTCAAATTAGACAACAAAGAAAACTGTGATAAAATTTTAGAGCACAAAAGATTGACAAAAGTGAACTCATTCGAGAAGAATAACGAAATCAAAGCAAGCAACAAAGCGATTGAAGATAACAAAGTCTTGAGTGATGCTCTGAAGAAAGAATTGACAGATTGTCCCAGCAGCGACAAAAAAGCTCAAAAGTTTCAATCCCTGAATAATGAGCTTTTGAATTTATTACAGGAAAAGACCTGTGTTAAACTTCGCCGTTCTGAAAGTTTGAACAAGCGCTCAGAGAAAGTTGACAACACCAGTCTAAAACTTCGTCGCTCCGAGTCTCTGAACAAGTATGACAAGTGTAAAAACATTGAGAACAGAATTTCAAAACTAGAGTTTCTCTCAAAAAATTGTGAAAATAAAGCCAACAACTTCAAAAGGTCCGACTCGTTAACAAAAACTGAAAAGACAGAATCAAACTTGATGAAGAGGAGGCAACAAGAGCTCTCTAAAAATCGGTGGTCGCGGGAAAAAGAGAACACGACAAAACTGAAAAGGAAAAACGGTATCTCGTCGAATAGGTCTATCAAACGGCGGCATACGGTGGGCGGAACAAAAGACTTTGACAAAATCAACTGGCTGGACAACAAG)(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAACTTCGTCGCTCCGAGT | GCCGCCGTTTGATAGACCTA | Start: 2837 |
| Tm | 59.968 | 59.896 | End: 3114 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | TGGTCGCGGGAAAAAGAGAA | GCTTGTTGTCCAGCCAGTTG | Start: 3036 |
| Tm | 59.893 | 59.969 | End: 3168 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | AAAACTTCGTCGCTCCGAGT | TTCTCTTTTTCCCGCGACCA | Start: 2837 |
| Tm | 59.968 | 59.893 | End: 3055 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | CGCGGGAAAAAGAGAACACG | GCTTGTTGTCCAGCCAGTTG | Start: 3040 |
| Tm | 60.111 | 59.969 | End: 3168 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
|---|
| Sequence | AAAACTTCGTCGCTCCGAGT | CGTGTTCTCTTTTTCCCGCG | Start: 2837 |
| Tm | 59.968 | 60.111 | End: 3059 |
| Size | 20 | 20 | 223 |
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