Primer Details for TRINITY_DN68154_c2_g1_i1 | |||
|---|---|---|---|
| (AAG)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGACGAAAGAGAAAAAGAAGGA | ACCTCATCCTTTCTCTCTGCC | Start: 22 |
| Tm | 57.837 | 59.163 | End: 201 |
| Size | 24 | 21 | 180 |
| Primer 2 details | |||
| Sequence | AGAGACGAAAGAGAAAAAGAAGGA | CCTCATCCTTTCTCTCTGCCT | Start: 22 |
| Tm | 57.837 | 58.882 | End: 200 |
| Size | 24 | 21 | 179 |
| Primer 3 details | |||
| Sequence | AGAGACGAAAGAGAAAAAGAAGGAA | ACCTCATCCTTTCTCTCTGCC | Start: 22 |
| Tm | 58.416 | 59.163 | End: 201 |
| Size | 25 | 21 | 180 |
| Primer 4 details | |||
| Sequence | AAGAGACGAAAGAGAAAAAGAAGGA | ACCTCATCCTTTCTCTCTGCC | Start: 21 |
| Tm | 58.416 | 59.163 | End: 201 |
| Size | 25 | 21 | 181 |
| Primer 5 details | |||
| Sequence | AGAAGAAGAAAGAGACGAAAGAGA | ACCTCATCCTTTCTCTCTGCC | Start: 12 |
| Tm | 57.357 | 59.163 | End: 201 |
| Size | 24 | 21 | 190 |
