ssr_primer
Primer Details for TRINITY_DN68129_c2_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGTCCAGGAGCACAAACA | GTTTTGGACCATAGTGCGCC | Start: 256 |
| Tm | 59.743 | 59.83 | End: 385 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | TGCAGCGTCGGATTCTTTCA | GTTTTGGACCATAGTGCGCC | Start: 195 |
| Tm | 60.32 | 59.83 | End: 385 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
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| Sequence | TGGTCCAGGAGCACAAACAT | GTTTTGGACCATAGTGCGCC | Start: 257 |
| Tm | 59.522 | 59.83 | End: 385 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
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| Sequence | TCGAATGAATCCGAGCAGCC | TGTTTGTGCTCCTGGACCAA | Start: 98 |
| Tm | 60.531 | 59.743 | End: 275 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
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| Sequence | TCGAATGAATCCGAGCAGCC | TGAAAGAATCCGACGCTGCA | Start: 98 |
| Tm | 60.531 | 60.32 | End: 214 |
| Size | 20 | 20 | 117 |
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