ssr_primer
Primer Details for TRINITY_DN68053_c4_g1_i6 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGCAGATCCCCGTCCTTA | CTCTTCCGATCTCGCCTTCC | Start: 1721 |
| Tm | 60.033 | 59.969 | End: 1857 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | GGTTTGCCCTTAAGCGGTTG | CTCTTCCGATCTCGCCTTCC | Start: 1696 |
| Tm | 60.039 | 59.969 | End: 1857 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | AAGCTCCTGGTCACGTCATG | GCACAAATTAAGCGCGGGAT | Start: 49 |
| Tm | 60.037 | 59.899 | End: 318 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | CAAGCTCCTGGTCACGTCAT | GCACAAATTAAGCGCGGGAT | Start: 48 |
| Tm | 60.037 | 59.899 | End: 318 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | ATCGCCTGGGCTATGTGAAG | CTCTTCCGATCTCGCCTTCC | Start: 1758 |
| Tm | 59.893 | 59.969 | End: 1857 |
| Size | 20 | 20 | 100 |
(A)11ttgagccgacgattttcagctctaaaagtaggtagtgcagtgtgttgggaattaaggcaagccacatttctcaataaaat(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGCAGATCCCCGTCCTTA | CTCTTCCGATCTCGCCTTCC | Start: 1721 |
| Tm | 60.033 | 59.969 | End: 1857 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | GGTTTGCCCTTAAGCGGTTG | CTCTTCCGATCTCGCCTTCC | Start: 1696 |
| Tm | 60.039 | 59.969 | End: 1857 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | AAGCTCCTGGTCACGTCATG | GCACAAATTAAGCGCGGGAT | Start: 49 |
| Tm | 60.037 | 59.899 | End: 318 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | CAAGCTCCTGGTCACGTCAT | GCACAAATTAAGCGCGGGAT | Start: 48 |
| Tm | 60.037 | 59.899 | End: 318 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | ATCGCCTGGGCTATGTGAAG | CTCTTCCGATCTCGCCTTCC | Start: 1758 |
| Tm | 59.893 | 59.969 | End: 1857 |
| Size | 20 | 20 | 100 |
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