ssr_primer
Primer Details for TRINITY_DN68023_c0_g5_i5 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCTTTCGCAAAGGCCCG | TGAAGACCAATCCCACACGG | Start: 251 |
| Tm | 59.969 | 59.964 | End: 451 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | CCTGTCTGGCGGAGTTTTCT | TGAAGACCAATCCCACACGG | Start: 237 |
| Tm | 59.965 | 59.964 | End: 451 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | CATTGTGGGATGGTGGACGA | TGGCAAAGTCTACGGCACAT | Start: 1083 |
| Tm | 60.036 | 59.964 | End: 1342 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | CATTGTGGGATGGTGGACGA | GATCGGAAACAGGTCTGGCA | Start: 1083 |
| Tm | 60.036 | 60.036 | End: 1357 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | AGAGTGCTCGACTTCCTTGC | TGGCAAAGTCTACGGCACAT | Start: 1111 |
| Tm | 60.038 | 59.964 | End: 1342 |
| Size | 20 | 20 | 232 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCTTTCGCAAAGGCCCG | TGAAGACCAATCCCACACGG | Start: 251 |
| Tm | 59.969 | 59.964 | End: 451 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | CCTGTCTGGCGGAGTTTTCT | TGAAGACCAATCCCACACGG | Start: 237 |
| Tm | 59.965 | 59.964 | End: 451 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | CATTGTGGGATGGTGGACGA | TGGCAAAGTCTACGGCACAT | Start: 1083 |
| Tm | 60.036 | 59.964 | End: 1342 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | CATTGTGGGATGGTGGACGA | GATCGGAAACAGGTCTGGCA | Start: 1083 |
| Tm | 60.036 | 60.036 | End: 1357 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | AGAGTGCTCGACTTCCTTGC | TGGCAAAGTCTACGGCACAT | Start: 1111 |
| Tm | 60.038 | 59.964 | End: 1342 |
| Size | 20 | 20 | 232 |
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