ssr_primer
Primer Details for TRINITY_DN67997_c3_g1_i5 |
|---|
| (G)40: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCTTCATCAGCAGCAGCA | ATAAGGACGGCGCAATCGAT | Start: 5183 |
| Tm | 59.964 | 59.966 | End: 5404 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | GTACATGAGCAGGGGTGCTT | TCGACCGTCTCATCTCTGGT | Start: 5966 |
| Tm | 60.035 | 60.035 | End: 6095 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | CAGAAGGGAGGGCAAGACAG | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3669 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | GGAGGGCAAGACAGAAGACC | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3663 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | GGGAGGGCAAGACAGAAGAC | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3664 |
| Size | 20 | 20 | 141 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCTTCATCAGCAGCAGCA | ATAAGGACGGCGCAATCGAT | Start: 5183 |
| Tm | 59.964 | 59.966 | End: 5404 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | GTACATGAGCAGGGGTGCTT | TCGACCGTCTCATCTCTGGT | Start: 5966 |
| Tm | 60.035 | 60.035 | End: 6095 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | CAGAAGGGAGGGCAAGACAG | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3669 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | GGAGGGCAAGACAGAAGACC | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3663 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | GGGAGGGCAAGACAGAAGAC | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3664 |
| Size | 20 | 20 | 141 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCTTCATCAGCAGCAGCA | ATAAGGACGGCGCAATCGAT | Start: 5183 |
| Tm | 59.964 | 59.966 | End: 5404 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | GTACATGAGCAGGGGTGCTT | TCGACCGTCTCATCTCTGGT | Start: 5966 |
| Tm | 60.035 | 60.035 | End: 6095 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | CAGAAGGGAGGGCAAGACAG | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3669 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | GGAGGGCAAGACAGAAGACC | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3663 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AAGGAAGAAGGGCTCACACG | GGGAGGGCAAGACAGAAGAC | Start: 3524 |
| Tm | 59.965 | 60.036 | End: 3664 |
| Size | 20 | 20 | 141 |
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