ssr_primer
Primer Details for TRINITY_DN67996_c2_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAGCTCGTTTTGGCAACG | CAAGGGACAAAGGCTCCAGA | Start: 81 |
| Tm | 59.971 | 59.598 | End: 288 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
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| Sequence | TCTGGAGCCTTTGTCCCTTG | ATTGGGAGAGTTGTGCGGAG | Start: 269 |
| Tm | 59.598 | 60.036 | End: 402 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | TCTGGAGCCTTTGTCCCTTG | AGCCTACCCAGATGGAGGTT | Start: 269 |
| Tm | 59.598 | 59.955 | End: 430 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
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| Sequence | CTTACCTCGGGGCGGAAATT | CAAGGGACAAAGGCTCCAGA | Start: 54 |
| Tm | 60.107 | 59.598 | End: 288 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
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| Sequence | TCACGATCCGGTGCACAATA | ATTGGGAGAGTTGTGCGGAG | Start: 152 |
| Tm | 59.467 | 60.036 | End: 402 |
| Size | 20 | 20 | 251 |
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