ssr_primer
Primer Details for TRINITY_DN67970_c5_g1_i3 |
|---|
| (TAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATCGCAGGGCATTTTGA | GCGTCGGTGGGTATTCGTAT | Start: 134 |
| Tm | 60.034 | 59.969 | End: 323 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | TGACACCTGCGGGATAAACC | GCGTCGGTGGGTATTCGTAT | Start: 151 |
| Tm | 60.036 | 59.969 | End: 323 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CAGGGCATTTTGACACCTGC | GCGTCGGTGGGTATTCGTAT | Start: 141 |
| Tm | 60.038 | 59.969 | End: 323 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
|---|
| Sequence | GGATCGCAGGGCATTTTGAC | GCGTCGGTGGGTATTCGTAT | Start: 135 |
| Tm | 59.899 | 59.969 | End: 323 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
|---|
| Sequence | ATACGAATACCCACCGACGC | CTGAAGCGATCCCCGAAAGT | Start: 304 |
| Tm | 59.969 | 60.108 | End: 413 |
| Size | 20 | 20 | 110 |
(T)37: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATCGCAGGGCATTTTGA | GCGTCGGTGGGTATTCGTAT | Start: 134 |
| Tm | 60.034 | 59.969 | End: 323 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | TGACACCTGCGGGATAAACC | GCGTCGGTGGGTATTCGTAT | Start: 151 |
| Tm | 60.036 | 59.969 | End: 323 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CAGGGCATTTTGACACCTGC | GCGTCGGTGGGTATTCGTAT | Start: 141 |
| Tm | 60.038 | 59.969 | End: 323 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
|---|
| Sequence | GGATCGCAGGGCATTTTGAC | GCGTCGGTGGGTATTCGTAT | Start: 135 |
| Tm | 59.899 | 59.969 | End: 323 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
|---|
| Sequence | ATACGAATACCCACCGACGC | CTGAAGCGATCCCCGAAAGT | Start: 304 |
| Tm | 59.969 | 60.108 | End: 413 |
| Size | 20 | 20 | 110 |
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