ssr_primer
Primer Details for TRINITY_DN67901_c5_g2_i10 |
|---|
| (T)26: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTCCCCTTCGTAGGTCTC | GTCGCTCGCAAGTGTGTTTT | Start: 1959 |
| Tm | 60.033 | 59.972 | End: 2068 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | CTGGTCCTCCGCTTGACTTT | GTCGCTCGCAAGTGTGTTTT | Start: 1932 |
| Tm | 59.965 | 59.972 | End: 2068 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | CTTTGGGCTGTGACGCTTTC | AGTCAAGCCACGCAAGATGA | Start: 1354 |
| Tm | 60.04 | 59.965 | End: 1507 |
| Size | 20 | 20 | 154 |
| Primer 4 details |
|---|
| Sequence | AAAACACACTTGCGAGCGAC | GTCGTAAGCTCGGTCGCTAA | Start: 2049 |
| Tm | 59.972 | 59.902 | End: 2251 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TCATCTTGCGTGGCTTGACT | GAGAAGAAGCCGACTGGACC | Start: 1488 |
| Tm | 59.965 | 60.109 | End: 1717 |
| Size | 20 | 20 | 230 |
(C)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTCCCCTTCGTAGGTCTC | GTCGCTCGCAAGTGTGTTTT | Start: 1959 |
| Tm | 60.033 | 59.972 | End: 2068 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | CTGGTCCTCCGCTTGACTTT | GTCGCTCGCAAGTGTGTTTT | Start: 1932 |
| Tm | 59.965 | 59.972 | End: 2068 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | CTTTGGGCTGTGACGCTTTC | AGTCAAGCCACGCAAGATGA | Start: 1354 |
| Tm | 60.04 | 59.965 | End: 1507 |
| Size | 20 | 20 | 154 |
| Primer 4 details |
|---|
| Sequence | AAAACACACTTGCGAGCGAC | GTCGTAAGCTCGGTCGCTAA | Start: 2049 |
| Tm | 59.972 | 59.902 | End: 2251 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TCATCTTGCGTGGCTTGACT | GAGAAGAAGCCGACTGGACC | Start: 1488 |
| Tm | 59.965 | 60.109 | End: 1717 |
| Size | 20 | 20 | 230 |
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