ssr_primer
Primer Details for TRINITY_DN67853_c5_g1_i1 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGTGCTCAGCTCAAGTC | AAGACGGGACAGATGCAGTG | Start: 1214 |
| Tm | 59.965 | 60.037 | End: 1397 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | CACTGCATCTGTCCCGTCTT | GGAGCCCTTCCTTTGTCGAA | Start: 1378 |
| Tm | 60.037 | 59.964 | End: 1521 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | ACGCGTTCGCTCAAAATACG | ACCGCGGATCTGGATCAATC | Start: 41 |
| Tm | 59.908 | 59.966 | End: 273 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
|---|
| Sequence | GGCGGGGCCTATTACTGTTT | AACACTGAATAGGGCGTGGG | Start: 1734 |
| Tm | 60.107 | 60.035 | End: 1975 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | AAGCGTCTTAGGGCACACAA | GGAGCCCTTCCTTTGTCGAA | Start: 1262 |
| Tm | 59.892 | 59.964 | End: 1521 |
| Size | 20 | 20 | 260 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGTGCTCAGCTCAAGTC | AAGACGGGACAGATGCAGTG | Start: 1214 |
| Tm | 59.965 | 60.037 | End: 1397 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | CACTGCATCTGTCCCGTCTT | GGAGCCCTTCCTTTGTCGAA | Start: 1378 |
| Tm | 60.037 | 59.964 | End: 1521 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | ACGCGTTCGCTCAAAATACG | ACCGCGGATCTGGATCAATC | Start: 41 |
| Tm | 59.908 | 59.966 | End: 273 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
|---|
| Sequence | GGCGGGGCCTATTACTGTTT | AACACTGAATAGGGCGTGGG | Start: 1734 |
| Tm | 60.107 | 60.035 | End: 1975 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | AAGCGTCTTAGGGCACACAA | GGAGCCCTTCCTTTGTCGAA | Start: 1262 |
| Tm | 59.892 | 59.964 | End: 1521 |
| Size | 20 | 20 | 260 |
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