ssr_primer
Primer Details for TRINITY_DN67844_c0_g1_i2 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAGCTGAAACACCTCCGC | GGCTTCCAACTGCCTTTTGG | Start: 228 |
| Tm | 60.037 | 59.966 | End: 488 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
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| Sequence | ACTAGCTGAAACACCTCCGC | TGGTGGCATCTTGAGCAAGT | Start: 228 |
| Tm | 60.037 | 59.889 | End: 471 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
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| Sequence | TCCCTTTGAACCTCCTCGGA | GGCTTCCAACTGCCTTTTGG | Start: 372 |
| Tm | 60.179 | 59.966 | End: 488 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
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| Sequence | ACTAGCTGAAACACCTCCGC | TCCGAGGAGGTTCAAAGGGA | Start: 228 |
| Tm | 60.037 | 60.179 | End: 391 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
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| Sequence | CGGGATCGCCGTATGAGAAT | GGCTTCCAACTGCCTTTTGG | Start: 314 |
| Tm | 59.756 | 59.966 | End: 488 |
| Size | 20 | 20 | 175 |
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