ssr_primer
Primer Details for TRINITY_DN67822_c11_g1_i1 |
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| (AGA)5aa(AAG)5aggtaggtggaagaagaagaagag(GAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGAAGAAGAAGAGGTAGGTGG | TCCTCTTCTTCTTCCTCTTCTTGC | Start: 50 |
| Tm | 59.225 | 60.021 | End: 149 |
| Size | 24 | 24 | 100 |
| Primer 2 details |
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| Sequence | AGGTGGAAGAAGAAGAAGAGGA | TCTCTTCCTCTTCTTCCTCTCT | Start: 68 |
| Tm | 58.137 | 57.054 | End: 244 |
| Size | 22 | 22 | 177 |
| Primer 3 details |
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| Sequence | AGCAAGAAGAGGAAGAAGAAGAGG | TCTCTTCCTCTTCTTCCTCTCT | Start: 125 |
| Tm | 60.02 | 57.054 | End: 244 |
| Size | 24 | 22 | 120 |
| Primer 4 details |
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| Sequence | GCAAGAAGAGGAAGAAGAAGAGGA | TCTCTTCCTCTTCTTCCTCTCT | Start: 126 |
| Tm | 60.021 | 57.054 | End: 244 |
| Size | 24 | 22 | 119 |
| Primer 5 details |
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| Sequence | GGTAGGTGGAAGAAGAAGAAGAGG | TCTCTTCCTCTTCTTCCTCTCT | Start: 65 |
| Tm | 60.081 | 57.054 | End: 244 |
| Size | 24 | 22 | 180 |
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