ssr_primer
Primer Details for TRINITY_DN67809_c4_g1_i7 |
|---|
| (T)44: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACTCAACCGACCAAGTG | TGGCTATGGACCGGAAGAGA | Start: 716 |
| Tm | 59.968 | 60.033 | End: 966 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | AACCGACCAAGTGTTTCCGT | TGGCTATGGACCGGAAGAGA | Start: 723 |
| Tm | 60.108 | 60.033 | End: 966 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | AGCAGTCACACATATGGGCC | AAAGCTCAGCACGTGTAGCT | Start: 1596 |
| Tm | 60.107 | 59.966 | End: 1802 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | TTGATTCCACCAGCCGTGAA | AAAGCTCAGCACGTGTAGCT | Start: 1572 |
| Tm | 59.89 | 59.966 | End: 1802 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
|---|
| Sequence | TTTGATTCCACCAGCCGTGA | AAAGCTCAGCACGTGTAGCT | Start: 1571 |
| Tm | 59.89 | 59.966 | End: 1802 |
| Size | 20 | 20 | 232 |
(T)16g(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACTCAACCGACCAAGTG | TGGCTATGGACCGGAAGAGA | Start: 716 |
| Tm | 59.968 | 60.033 | End: 966 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | AACCGACCAAGTGTTTCCGT | TGGCTATGGACCGGAAGAGA | Start: 723 |
| Tm | 60.108 | 60.033 | End: 966 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | AGCAGTCACACATATGGGCC | AAAGCTCAGCACGTGTAGCT | Start: 1596 |
| Tm | 60.107 | 59.966 | End: 1802 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | TTGATTCCACCAGCCGTGAA | AAAGCTCAGCACGTGTAGCT | Start: 1572 |
| Tm | 59.89 | 59.966 | End: 1802 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
|---|
| Sequence | TTTGATTCCACCAGCCGTGA | AAAGCTCAGCACGTGTAGCT | Start: 1571 |
| Tm | 59.89 | 59.966 | End: 1802 |
| Size | 20 | 20 | 232 |
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