ssr_primer
Primer Details for TRINITY_DN67775_c1_g2_i1 |
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| (A)16tcccccc(A)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATTCGCCCCTAAGAGAGG | GAGCTCTCTTGGCGTCTTCA | Start: 172 |
| Tm | 59.929 | 59.755 | End: 311 |
| Size | 21 | 20 | 140 |
| Primer 2 details |
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| Sequence | GGAATTCGCCCCTAAGAGAGG | TTCTGACGAGCTCTCTTGGC | Start: 172 |
| Tm | 59.929 | 59.755 | End: 318 |
| Size | 21 | 20 | 147 |
| Primer 3 details |
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| Sequence | GCCAAGAGAGCTCGTCAGAA | GCATTGCCGTGCCAATAAAGA | Start: 299 |
| Tm | 59.755 | 60.135 | End: 428 |
| Size | 20 | 21 | 130 |
| Primer 4 details |
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| Sequence | TGAAGACGCCAAGAGAGCTC | GCATTGCCGTGCCAATAAAGA | Start: 292 |
| Tm | 59.755 | 60.135 | End: 428 |
| Size | 20 | 21 | 137 |
| Primer 5 details |
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| Sequence | GCCAAGAGAGCTCGTCAGAA | GCATTGCCGTGCCAATAAAG | Start: 299 |
| Tm | 59.755 | 58.72 | End: 428 |
| Size | 20 | 20 | 130 |
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