ssr_primer
Primer Details for TRINITY_DN67766_c2_g1_i1 |
|---|
| (T)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGGCCTGGGTGGATAT | TACGAGGGTAACGTAGGCCA | Start: 1196 |
| Tm | 60.029 | 60.035 | End: 1331 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGCCTCAGCAGACCAATCAG | Start: 1196 |
| Tm | 60.029 | 60.036 | End: 1417 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGTACGCAGAGGCATTGGTT | Start: 1196 |
| Tm | 60.029 | 59.964 | End: 1372 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | ACCAATCAGAGTGACTGCGG | Start: 1196 |
| Tm | 60.029 | 60.037 | End: 1406 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | TGGCCTACGTTACCCTCGTA | TGATAGCACGGCCAACATGT | Start: 1312 |
| Tm | 60.035 | 60.036 | End: 1509 |
| Size | 20 | 20 | 198 |
(T)11ccctccgtttttgttttacccc(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGGCCTGGGTGGATAT | TACGAGGGTAACGTAGGCCA | Start: 1196 |
| Tm | 60.029 | 60.035 | End: 1331 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGCCTCAGCAGACCAATCAG | Start: 1196 |
| Tm | 60.029 | 60.036 | End: 1417 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGTACGCAGAGGCATTGGTT | Start: 1196 |
| Tm | 60.029 | 59.964 | End: 1372 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | ACCAATCAGAGTGACTGCGG | Start: 1196 |
| Tm | 60.029 | 60.037 | End: 1406 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | TGGCCTACGTTACCCTCGTA | TGATAGCACGGCCAACATGT | Start: 1312 |
| Tm | 60.035 | 60.036 | End: 1509 |
| Size | 20 | 20 | 198 |
(C)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGGCCTGGGTGGATAT | TACGAGGGTAACGTAGGCCA | Start: 1196 |
| Tm | 60.029 | 60.035 | End: 1331 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGCCTCAGCAGACCAATCAG | Start: 1196 |
| Tm | 60.029 | 60.036 | End: 1417 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGTACGCAGAGGCATTGGTT | Start: 1196 |
| Tm | 60.029 | 59.964 | End: 1372 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | ACCAATCAGAGTGACTGCGG | Start: 1196 |
| Tm | 60.029 | 60.037 | End: 1406 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | TGGCCTACGTTACCCTCGTA | TGATAGCACGGCCAACATGT | Start: 1312 |
| Tm | 60.035 | 60.036 | End: 1509 |
| Size | 20 | 20 | 198 |
(C)11tca(AT)10(T)35*: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGGCCTGGGTGGATAT | TACGAGGGTAACGTAGGCCA | Start: 1196 |
| Tm | 60.029 | 60.035 | End: 1331 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGCCTCAGCAGACCAATCAG | Start: 1196 |
| Tm | 60.029 | 60.036 | End: 1417 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGTACGCAGAGGCATTGGTT | Start: 1196 |
| Tm | 60.029 | 59.964 | End: 1372 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | ACCAATCAGAGTGACTGCGG | Start: 1196 |
| Tm | 60.029 | 60.037 | End: 1406 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | TGGCCTACGTTACCCTCGTA | TGATAGCACGGCCAACATGT | Start: 1312 |
| Tm | 60.035 | 60.036 | End: 1509 |
| Size | 20 | 20 | 198 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGGCCTGGGTGGATAT | TACGAGGGTAACGTAGGCCA | Start: 1196 |
| Tm | 60.029 | 60.035 | End: 1331 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGCCTCAGCAGACCAATCAG | Start: 1196 |
| Tm | 60.029 | 60.036 | End: 1417 |
| Size | 20 | 20 | 222 |
| Primer 3 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | TGTACGCAGAGGCATTGGTT | Start: 1196 |
| Tm | 60.029 | 59.964 | End: 1372 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | AGGAAGGCCTGGGTGGATAT | ACCAATCAGAGTGACTGCGG | Start: 1196 |
| Tm | 60.029 | 60.037 | End: 1406 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | TGGCCTACGTTACCCTCGTA | TGATAGCACGGCCAACATGT | Start: 1312 |
| Tm | 60.035 | 60.036 | End: 1509 |
| Size | 20 | 20 | 198 |
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