ssr_primer
Primer Details for TRINITY_DN67753_c0_g1_i6 |
|---|
| (G)12ttcaattcg(T)28: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGATTGTGCTCGTCTCGG | ACCATGCACAGTCTCTTCGG | Start: 506 |
| Tm | 60.249 | 60.037 | End: 676 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | ATCGATTGTGCTCGTCTCGG | CGTCACCATGCACAGTCTCT | Start: 506 |
| Tm | 60.249 | 60.038 | End: 680 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | TATGAAGGTTGGGGTGGGGA | CCGAGACGAGCACAATCGAT | Start: 370 |
| Tm | 60.179 | 60.249 | End: 525 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | ACCTATTCCGAGGGAGCGAA | ACCATGCACAGTCTCTTCGG | Start: 474 |
| Tm | 60.397 | 60.037 | End: 676 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | AACCTATTCCGAGGGAGCGA | ACCATGCACAGTCTCTTCGG | Start: 473 |
| Tm | 60.397 | 60.037 | End: 676 |
| Size | 20 | 20 | 204 |
(G)29: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGATTGTGCTCGTCTCGG | ACCATGCACAGTCTCTTCGG | Start: 506 |
| Tm | 60.249 | 60.037 | End: 676 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | ATCGATTGTGCTCGTCTCGG | CGTCACCATGCACAGTCTCT | Start: 506 |
| Tm | 60.249 | 60.038 | End: 680 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | TATGAAGGTTGGGGTGGGGA | CCGAGACGAGCACAATCGAT | Start: 370 |
| Tm | 60.179 | 60.249 | End: 525 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | ACCTATTCCGAGGGAGCGAA | ACCATGCACAGTCTCTTCGG | Start: 474 |
| Tm | 60.397 | 60.037 | End: 676 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | AACCTATTCCGAGGGAGCGA | ACCATGCACAGTCTCTTCGG | Start: 473 |
| Tm | 60.397 | 60.037 | End: 676 |
| Size | 20 | 20 | 204 |
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